2014   Exhibitors   Product Workshops

As part of the meeting program, exhibitors will have an opportunity to host a Product Workshop. Space for exhibitor workshops may be obtained at a cost of $1,500 per 1-hour session (which includes a lectern, lectern microphone, LCD projector, screen, laser pointer, easel, one 6' table inside the room, one 6' table outside the room and one complimentary mailing list of attendees). Exhibitors are responsible for providing their own computer.

Workshop applications will be reviewed and considered on a first-come, first-serve basis. The deadline to have a Product Workshop listed in the Meeting Program will be January 21, 2014. Workshop applications received after January 8 will be listed in the Meeting Addendum. Deadline for Meeting Addendum is March 5, 2014.

Government Agencies also have the opportunity to host a workshop at the discounted rate of $500 per 1-hour session (including equipment mentioned above).

Product Workshop Application

Sunday, April 27

Avanti Lipid Tools: Connecting Lipids, Technology and Cell Biology
Avanti Polar Lipids, Inc.
700 Industrial Park Drive
Alabaster, AL  35007
Phone: 205-663-2494
Fax:  205-663-0756
Email: kacee.sims@avantilipids.com
Web: www.avantilipids.com
10:00 AM – 11:00 AM – Room A (Hall A) – Show Floor
Presenter:  Kacee Sims, Ph.D.

Lipid research is challenging owing to the complexity and diversity of the lipidome. This seminar will focus on a set of experimental tools developed for the seasoned lipid researcher, as well as those new to the field of lipid research. Novel tools for probing protein-lipid interactions, applications for lipid binding antibodies, enhanced systems for the cellular delivery of lipids, improved visualization of lipid membranes using gold-labeled lipids, and advances in mass spectrometric analysis techniques will be discussed. Because lipid mediators are known to participate in a host of signal transduction and trafficking pathways within the cell, a comprehensive lipid toolbox that aids the science of lipidomics research is essential to better understand the molecular mechanisms of interactions between cellular components.

BIO Principles – Innovation and Value in Biology
John Wiley & Sons
111 River Street
Hoboken, NJ  07030
Phone: 201-748-6548
Web: www.wiley.com
11:00 AM – 12:00 PM – Room B (Hall A) – Show Floor
Presenters:  Clay Stone; Bonnie Roth

Are you interested in exploring a solution for your Majors Biology course that is truly innovative? You may have heard about OpenStax College’s exciting initiatives involving development of free online open-source textbooks based on high quality, peer-reviewed content. Wiley’s BIO –Principles includes the OpenStax open-source majors biology text, delivered through the new WileyPLUS Learning Space, an adaptive and collaborative teaching and learning environment. BIO Principles aims to: (bullet)Increase student engagement by providing a personalized learning experience, with resources that facilitate students owning their study time so they can come to class prepared for higher-level learning (bullet) Provide valuable content and services to foster critical thinking about course content (bullet) Support a variety of teaching and learning styles In our session, BIO Principles – Innovation and Value in Biology, you will participate in a discussion with a small group of other majors biology instructors about your course; opportunities and obstacles in our changing educational environment, and provide early feedback on BIO Principles.
What’s New in EndNote®
Thomson Reuters
1500 Spring Garden Street, 4th Floor
Philadelphia, PA  19130
Phone: 215-823-5327
Web: www.thomsonreuters.com
2:00 PM – 3:00 PM – Room A (Hall A) – Show Floor
Presentes: Meredith McCusker

EndNote® has always been the most powerful tool for managing your research. Whether it’s for maintaining your CV, expediting funding applications, or writing a manuscript, EndNote will help reduce the time you spend searching, updating, and organizing your research and formatting documents. But, EndNote is for more than just bibliographies. In 2014, we are releasing new tools and features that will transform the way researchers work. Based on feedback from users worldwide, we are building better team workflow solutions, robust collaboration features, and expanded mobile options, just to name a few. Join us for a first look at what’s coming for EndNote in 2014.
Monday, April 28

Easy and Automated Purification of His and GST Tagged Proteins Using ÄKTA™ start

GE Healthcare
800 Centennial Avenue
Piscataway, NJ  08854
Phone: 800-526-3593 or 732-457-8000
Fax:  877-295-8102
Email: cs-us@ge.com
Web:  www.gelifesciences.com
9:00 AM – 10:00 AM – Room A (Hall A) – Show Floor

Expression of recombinant protein using affinity tags is a commonly used purification strategy. Histidine and glutathione-S transferase(GST are the most common and versatile of these tags. Histidine tags are small and therefore less disruptive to the protein structures and functions. GST tags enhance solubility of the target protein, hence improving yield. However, GST tags are larger and their removal from target proteins is often necessary. Manual purification of tagged proteins often faces issues with time consumption, reproducibility, poor yield and purity etc. Other challenges might include column packing, sample loading/collection and measuring of protein concentration. ÄKTA start is a newly-developed laboratory scale protein purification system that can address some of these purification challenges. We will show that this approach provided high yields and consistent purities. Join us to learn how to automate your purification of His and GST tagged proteins using ÄKTA start.

Optimizing Sample Preparation

Labconco Corporation
8811 Prospect Avenue
Kansas City, MO  64132
Phone: 816-333-8811
Email: Labconco@labconco.com
Web:  www.labconco.com

9:00 AM – 10:00 AM – Room B (Hall A) – Show Floor
Presenter:  Jenny Sprung
This intermediate level class addresses many of the different ways to prepare a sample for analysis. Concentration methods all have their benefits and pitfalls. Lyophilization, vacuum concentration and vacuum evaporation will all be covered. How do you chose the best method for your application? How can you maximize your results?
Coming soon…..NEW Amersham™ 600 Imager series (CCD-based Camera Systems)

GE Healthcare Life Sciences
800 Centennial Avenue
Piscataway, NJ  08854
Phone: 800-526-3593 or 732-457-8000
Fax:  877-295-8102
Email: cs-us@ge.com
Web:  www.gelifesciences.com

10:30 AM – 11:30 AM – Room A (Hall A) – Show Floor

Join us to learn more about the newest generation of imaging systems! The Amersham™ 600 series builds upon the robust legacy of ImageQuant™ LAS4000 series that provided high quality, publication-ready images and reliable research data. Amersham 600 portfolio includes four systems for routine research applications in pharmaceutical, biotechnology and academic research including Chemiluminescent and Fluorescent Western blotting, Gel documentation, DNA and RNA analysis, 2D gels and Colorimetric detection for quantitative and QC analysis. The new platform complements our Western blotting detection reagents, such as our legacy Amersham™ ECL as well as Amersham ECL Prime, ECL Select and ECL Plex to provide a complete Western blotting solution. Please join us to learn more about how our new CCD-based camera systems can help you in your research!

Can We Trust Western Blots?

Bio-Rad Laboratories
2000 Alfred Nobel Drive
Hercules, CA  94547
Phone: 510-741-5052
Fax:  510-741-5630
Web:  www.bio-rad.com
12:00 PM – 1:00 PM – Room A (Hall A) – Show Floor

Presenters:  Aldrin V. Gomes, Department of Neurobiology, Physiology, and Behavior, University of California, Davis, CA

Western blotting is a widely used technique to identify and semi-quantify a protein in a complex sample. Unfortunately, as an editor of several journals, I routinely observe Western blots which are not properly conducted. The low quality Western blot data have led scientists to question whether we can trust the quantification of proteins by using Western blots. Reducing errors and misinterpretations requires standardized procedure and proper controls. In this talk I will discuss the following key factors for high confident western blot.

Surgical Monitoring: Generate Consistent Surgical Outcomes & Satisfy your IACUC with the MouseMonitor™ S
Indus Instruments
721 Tristar Drive
Webster, TX  77598
Phone: 281-286-1130
Fax:  281-461-8168
Email: gsattler@indusinstruments.com
Web:  www.indusinstruments.com

10:30 AM – 11:30 AM – Room B (Hall A) – Show Floor

Presenter: Graham Sattler
It takes high quality data to publish the kind of quality science that will get you noticed.  With so many experimental variables at play, getting the data you need can be a challenge.  Comprehensive surgical monitoring goes a long way toward improving the surgical consistency of the subjects in your study group and ensures that your conclusions are based on consistent results.  Continuous monitoring during surgery provides you with the information you need to make informed decisions in the moment as well as the data you need to document your studies for collaboration and publishing.  Implementing a comprehensive heating and surgical monitoring solution helps you adhere to all of the recommendations in the NRC’s Guide for the Care and Use of Laboratory Animals, 8th edition, and helps your protocols meet increased IACUC monitoring requirements.  Join Graham Sattler of Indus Instruments to learn more about how implementing a surgical monitoring program with the MouseMonitor™ S in your lab can help improve surgical consistency, document your studies and help keep you in your IACUC’s “good book”.  Topics range from the importance of heating and temperature monitoring to the need for monitoring heart rate, respiration rate and blood oxygenation to insure proper sedation and adequate ventilation.  Learn more about how an integrated approach to surgical monitoring can reduce both your workload during surgery and the number of surgeries that you need to perform. Surgical monitoring is key to increased surgical consistency and more successful surgical outcomes; take advantage of this workshop to learn more about surgical monitoring with the MouseMonitor™ S and its many benefits.

From Western Blotting to Imaging: Novel Approaches to Microplate Reader Applications
Molecular Devices LLC
1311 Orleans Drive
Sunnyvale, CA  94089
Phone: 408-747-1700
Fax:  408-548-6431
Email:  info@moldev.com
Web:  www.moleculardevices.com

1:30 PM – 2:30 PM – Room A (Hall A) – Show Floor

Presenters:  Cathy Olsen

During this workshop, you will learn how you can run all of these applications (and more) on a multi-mode microplate reader: • Western blotting with improved stability and quantitation • StainFree™ transmitted light analysis and fluorescent cellular imaging • Screening assays including fluorescence polarization and TR-FRET • Monitoring cytotoxicity using multiplexed cell based assays • Low volume quantitation of nucleic acids and proteins.

Bruker Corporation
40 Manning Road
Billerica, MA  01821
Phone: 978-663-3660
Email: ms-sales@bdal.com
Web: www.bruker.com
12:00 PM – 1:00 PM – Room B – (Hall A) Show Floor
1)  Advances in Multimodal Imaging Technologies –Cutting Edge Strategies in Gamma Detector Technology and a New Architecture for Optical/X-Ray Imaging

Presenter:  Dr. Andrew Van Praagh, PhD

Multimodal imaging devices can allow preclinical investigators to pursue multifaceted examinations of whole animal model systems, non-invasively.  Animal organ/tissue physiology and structure, and the PK/PD characteristics of candidate therapeutics may be rapidly evaluated.  This presentation will introduce two multimodal imaging systems from Bruker:  The Albira and the In-vivo Xtreme.  The Albira is a revolutionary PET/SPECT/CT trimodal system with proprietary, single-crystal PET and SPECT detector technology, that resolves depth of interaction (DOI) and Parralax Error issues.  The In-vivo Xtreme can acquire and co-register fluorescent, luminescent, radioisotopic, and/or digital X-Ray images.  Albira and the In-vivo Xtreme imaging versatility will be shown by presenting successful application studies  in disciplines including oncology, neuroscience, arthritis, microbial infection, and blunt force trauma.

2)  Imaging Mass Spectrometry-A Powerful Hypothesis Generating Tool for Investigating Microbial Systems

  Vanessa Phelan, Univeristy of California San Diego
visualization of microbial molecules in IMS allows us to quickly gauge changes in metabolite production due to external stimuli and genetic changes.   This presentation will introduce microbial IMS, highlight sample preparation and discuss experimental challenges.  Finally, examples of successful application of microbial IMS will be shown including uncovering inter-kingdom biotransformations, hidden chemotypes of phenotypically similar interactions and potential new therapeutics against microbial pathogens.

New Rodent Anesthesia Method with Physiological Monitoring

Kent Scientific
1116 Litchfield Street
Torrington, CT  06790
Phone: 860-626-1172
Fax: 860-626-1179
Email: sales@kentscientific.com
Web: www.kentscientific.com

1:30 PM – 2:30 PM, Room B, Show Floor – (Hall A) Show Floor

Presenter: Andrew Ide
Anesthesia is a complex procedure that introduces variables in your study. Biomedical research involves eliminating variables so you can focus on your study objective, produce valid/reproducible data and publish your research. The normal physiological condition of rodents under anesthesia is altered by the introduction of the anesthetic. Subsequent surgical procedures and other activities performed while the animal is under can further change the physiological state of the rodent. Any change in temperature, oxygen saturation, respiratory rate, heart rate or blood pressure introduces a variable into your study that needs to be controlled. Precise delivery and control of inhalation anesthetics is critically important to the safety of the animal and the researcher. Reducing the amount of anesthetic used greatly reduces exposure to the researcher. In this workshop you will learn about what effects anesthetics have on rodents, what variables you should monitor during anesthesia and how to effectively incorporate surgical monitoring into your protocol. You will also learn how to reduce your risk of exposure to inhalation anesthetics by using a digitally-controlled vaporizer that is safer for you and your animal.

New Features in Origin 9.1 - Data Analysis and Graphing Software

OriginLab Corporation
One Roundhouse Plaza – Suite 303
Northampton, MA  01060
Phone: 413-586-2013
Fax: 413-585-0126
Email: sales@originlab.com
Web: www.originlab.com

3:00 PM – 4:00 PM, Room B, Show Floor – (Hall A) Show Floor

Presenter:  Snow Li

OriginLab technical staff will demonstrate new features and improvements in the latest version of Origin and OriginPro. The demonstration will be followed by a Q&A session.


Tuesday, April 29

The ZDF Rat as a Model Type 2 Diabetes
Charles River
251 Ballardvale Street
Wilmington, MA  01887
Phone: 978-835-6781
Web: www.crl.com
11:00 AM – 12:00 PM – Room B (Hall A) – Show Floor
Presenter: Joe Cornicelli, Ph.D.
The incidence of obesity and diabetes is reaching near epidemic proportions worldwide. Studies in appropriate animal models are very important in order to gain a better understanding of these diseases. Historically, rodents have been a mainstay of diabetes research, various mouse and rat models have been described that mimic various aspects of obesity and obesity-driven diabetes. The ZDF rat is one such model. Because it is obese, hyperinsulinemic, and develops diet-dependent diabetes, The ZDF rat embodies many of the facets of human disease. This presentation explores the development of the diabetic phenotype in the ZDF rat. We also demonstrate effects of standard anti-diabetic treatments on glucose homeostasis and relevant biomarkers, as well as the effect of varying fasting times on glucose disposal.
Single Cell Western Blot and Stain-Free Total Protein Loading Control
Bio-Rad Laboratories
2000 Alfred Nobel Drive
Hercules, CA  94547
Phone: 510-741-5052
Fax:  510-741-5630
Web: www.bio-rad.com
12:00 PM – 1:00 PM – Room A (Hall A) – Show Floor
Presenter: Robyn M. Murphy, La Trobe University, Melbourne, Victoria 3086, Australia
Western blotting has been used for protein analyses in a wide range of tissue samples for more than 30 years.  Over the past few years, I have used skeletal muscle to develop a protocol for quantitatively analysing proteins in very small samples sizes, being 1-3 mm segments of skeletal muscle fibres (cells).  This amounts to 5-10 μg total mass of tissue or 1-3 μg total protein.  Through the development of this technique, it has become apparent that using very small amounts of representative sample presents the most optimal situation for analysing proteins. The introduction of Stain Free gels has allowed the methodology to advance, whereby an assessment of total protein can be attained during the normal western blotting protocol.  When combined with a calibration curve, quantitative assessment is achievable.  This talk will demonstrate how western blotting is reliable and quantitative, and hence remains extremely valuable for making biological measurements.