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As part of the meeting program, exhibitors will have an opportunity to host a Product Workshop. Space for exhibitor workshops may be obtained at a cost of $1,500 per 1-hour session (which includes a lectern, lectern microphone, LCD projector, screen, laser pointer, easel, one 6' table inside the room, one 6' table outside the room and one complimentary mailing list of attendees). Exhibitors are responsible for providing their own computer. Workshop applications will be reviewed and considered on a first-come, first-serve basis. The deadline to have a Product Workshop listed in the Meeting Program will be January 10, 2012. Workshop applications received after January 10 will be listed in the Meeting Addendum. Deadline for Meeting Addendum is March 6, 2012. Government Agencies also have the opportunity to host a workshop at the discounted rate of $500 per 1-hour session (including equipment mentioned above). Product Workshop Application
PRODUCT WORKSHOPS Sunday, April 22 A Fully Implantable Telemetry System to Measure Transit Time Blood Flow, Multiple Pressures, ECG and Temperature in NHP, Canines and Swine
Konigsberg Instruments
2000 East Foothill Boulevard
Pasadena, CA 91107
Phone: 626 585-4032
Fax: 626 585-4068
Email: w.j.mills@konigsberginc.com
Web: www.itstelemetry.com 10:00 A.M. – 11:00 A.M. – Room A (Hall A) – Show Floor Presenter: Steve Pettinger A discussion of the research applications and surgical techniques developed for the use of a revolutionary new fully implantable telemetry system that will measure Cardiac Output and Peripheral Blood Flow using Transit Time technology. The new T27 series encoder coupled with the TU7 implantable flow-meter permits full cardiac analysis in non-human primates, canines and swine. The battery life exceeds one year of continuous operation. A New Generation of Bruker AFM's for Biophysicists Bruker Corporation
112 Robin Hill Rd.
Santa Barbara, CA 93117
Email: cecilia.stenger@bruker-nano-com
Web: www.bruker-axs.com 1:00 P.M. – 2:00 P.M. – Room B (Hall A) – Show Floor Presenter: James E. Shaw, Ph.D. In this tutorial Bruker will present the latest advances in their full-line of atomic force microscopes (AFM) that continue to put their AFM systems at the forefront of biophysical research. Data will be presented from various application areas demonstrating the use of the self-optimizing ScanAsyst® Mode for ultra-low force high-resolution imaging of soft biological samples and PeakForce QNM® for correlative spatial mapping of sample nano-mechanical properties. We will present the latest in functionally integrated AFM and optical microscopy for live cell studies. We will also discuss how Bruker’s advances in high-speed AFM imaging are improving research productivity and providing biophysicists with unprecedented opportunities to directly observe the dynamics of biologically-related processes. EndoGear: A Fully Implantable Biotelemetry System to Measure Flow Velocity, Pressure and ECG in Cardiovascular Research Models Transonic Systems
34 Dutch Mill Road
Ithaca, NY 14850
Phone: 607 257-5300
Fax: 607 257-7256
Email: support@transonic.com
Web: www.transonic.com 1:00 P.M. – 2:00 P.M. – Room B (Hall A) – Show Floor Presenters: Koullis Pitsillides & Ghassan Kassab Telemetry is an established technique for obtaining valuable data from unrestrained animals both in the laboratory and the field. Telemetry systems that can measure simple data including ECG and blood pressure using fluid-filled catheters have previously been available, but telemetry systems that can provide measurements of both blood pressure and blood flow dynamics have only recently been introduced. Such implantable telemetry systems can provide a more complete picture of the physiological status of the animal model under investigation.
EndoGear is a new, fully implantable biotelemetry system that measures the critical parameters for a complete hemodynamic study profile: blood flow velocity using Doppler ultrasound technology, arterial and ventricular pressure using miniature solid-state pressure catheters, ECG and temperature.
This workshop will introduce the unique capabilities of these implants, discuss details of the sensors used, identify and describe animal model applications where combined blood flow velocity and blood pressure measurements are key considerations and examine some of the data obtained using the EndoGear system. Monday, April 23
Genetically Modified Cell Lines to Drive Your Research: Targeted Genome Editing with ZFNs to Create Powerful New Cellular Models Sigma Life Science
3050 Spruce St.
St. Louis, MO 63103
Phone: 314 771-5765
Email: brad.keller@sial.cm
Web: www.sigmaaldrich.com/life-science/cells-and-based-assays.html 12 Noon – 1:00 P.M. – Room B (Hall A) – Show Floor Presenter: Dr. Bradley T. Keller Zinc finger nucleases allow rapid and permanent modification of gene loci to construct gene knockouts, targeted knock-ins or reporter-tagged cell lines. Using ZFN technology, we are creating in vitro models with human cancer cells that have specific mutations for colon, lung and breast cancers. These cells contain patient-relevant mutations of disease-specific genes providing ideal models to study an individual's drug sensitivity and resistance, e.g., personalized medicine. ZFNs are also used to modify cellular genes with fluorescent reporters, thus facilitating live-cell imaging of tagged proteins at endogenous expression levels. This allows evaluation of complex protein dynamics in processes such as receptor-mediated endocytosis. In addition to off-the-shelf cell lines, Sigma’s Cell Design Studio uses ZFN technology to create custom genome modifications in mammalian cell lines tailored to highly specific research needs. Thus, our exciting cell line portfolio offers customers the perfect tools for their particular research applications. Utilizing Pressure-Volume Loops and Coronary Blood Flow to Assess Cardiac Efficiency in Miniature Swine with Compensated Heart Failure Following Low-Intensity Interval Exercise Scisense Systems Inc.
3397 White Oak Road, Unit 3
London, Ontario
Phone: 519 680-7677
Email: news@scisense.com
Web: www.scisense.com 1:00 P.M. – 2:00 P.M. – Room A (Hall A) – Show Floor Presenter: Dr. Craig A. Emter, Ph.D.-Dept. of Biomedical Sciences & Medical Pharmacology and Physiology
at the University of Missouri - Columbia Despite decades of research, the exact mechanisms underlying changes in coronary vascular function during the development of heart failure and its subsequent impact on metabolic and LV function have yet to be clearly determined. In this workshop, Dr. Craig Emter will explain how he uses PV loop and flow measurements in a complimentary manner in his own lab to better understand cardiac physiology in vivo. Ensuring Accuracy in Multiplex Immunoassay Results R&D Systems, Inc.
614 McKinley Place NE
Minneapolis, MN 55413
Phone: 612 379-2956
Fax: 612 379-6580
Email: Info@RnDSystems.com
Web: www.RnDSystems.com 2:00 P.M. – 3:00 P.M. – Room B (Hall A) – Show Floor Presenter: Jane Schmidt Multiplex immunoassays are an efficient tool for evaluating multiple biomarkers simultaneously. With multiplex technology, a single assay using less than 100 µL of sample can generate results that, with a traditional ELISA, would require several mL of sample and weeks of experimentation. However, one must keep in mind that the potential for cross-reactivity or interference in these assays also multiplies with the complexity of the panel. Multiplex assays must be fully validated to ensure accurate, precise, and reproducible results for the user. R&D Systems has decades of experience developing immunoassays that are recognized as the best available. In this workshop we will discuss some of the factors that can negatively impact the performance of an immunoassay and how these can be magnified in a multiplex experiment. We will discuss practical ways to evaluate the performance of a multiplex immunoassay, including calibration, spike recovery, dilution linearity, sensitivity, precision and specificity, and how to evaluate product literature. We will also discuss how to assess your results against the current knowledge of the biomarker itself. The Digital Journey: A Microscope to Publication Image Primer The Histochemical Society
P.O. 85630
Seattle, WA 98145-1630
Phone: 206 832-9853
Fax: 426 483-1058
Email: mmcgough@histochemicalsociety.org
Web: www.histochemicalsociety.org 4:00 P.M. – 5:00 P.M. – Room B (Hall A) – Show Floor Presenter: Jerry Sedgewick Moving from mounted labeled specimens on a microscope slide to publication quality images requires skills in three areas: Microscopy, Digital Photography and Post-Processing. These skills are usually taught independent of each other but this tutorial will follow the entire workflow from a fluorescently labeled microscope slide to a publication quality image. We include learning about the components of a microscope and how to take advantage of microscope features; which settings in camera software are most important; and the post-processing steps done in Photoshop© or in similar programs. This course is taught by a microscopist, who authored two books on scientific imaging and who directed a core light microscopy facility at the University of Minnesota for 15 years.
Tuesday, April 23
Electric Cell-Substrate Impedance Sensing: A Label-Free, Non-Invasive Method of Cell Measurement
Applied Biophysics
185 Jordon Road
Troy, MY 12180
Phone: 518-880-6860
Email: info@biophysics.com
Web: www.biophysics.com 10:00 A.M. – 11:00 A.M. – Room A (Hall A) – Show Floor Presenter: Chrisitian Renken This workshop will provide an overview of the use of impedance to detect cell morphological changes. Emphasis will be placed on the use of different AC frequencies to distinguish cell parameters. Applications include barrier function, permeability, invasion/extravasation, signal transduction, and proliferation. Knockout Rat Models from SAGE Labs to Drive Research of Cholesterol Disequilibria Sigma Life Science/SAGE Labs
3050 Spruce St.
St. Louis, MO 63103
Phone: 314 771-5765
Email: jeff.xue@sial.com
Web: www.sagereasearchmodels.com 12 Noon – 1:00 P.M. – Room A (Hall A) – Show Floor Presenter: Jeff S. Xue, PhD Cholesterol homeostasis has been correlated to several human disease pathologies including obesity, diabetes, atherosclerosis, Alzheimer’s disease and Huntington’s disease. To develop a better understanding of the mechanisms of cholesterol regulation we have created rats with manipulations in the genes Apoe, Ldlr and Lep. These mutants were created by injection of zinc finger nucleases (ZFN) into one-cell Sprague-Dawley rat embryos. Dimerization of ZFN pairs about their target DNA locus allows for FOKI nuclease activity, creating a double stranded break (DSB). DSBs are most commonly repaired by a mechanism called non-homologous end joining (NHEJ), which functions at varying fidelity. This introduces frameshift mutations and leads to a lack of functional protein. Phenotypic characterization of homozygous null animals demonstrated high serum choleseterol level and insulin resistance at early age. The three knockout rat models developed at SAGE Labs will be helpful in the study of the etiology of several human pathologies related to cholesterol homeostasis.
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